1',5'-ANHYDROHEXITOL OLIGONUCLEOTIDES - HYBRIDIZATION AND STRAND DISPLACEMENT WITH OLIGORIBONUCLEOTIDES, INTERACTION WITH RNASE-H AND HIV REVERSE-TRANSCRIPTASE

Citation
C. Hendrix et al., 1',5'-ANHYDROHEXITOL OLIGONUCLEOTIDES - HYBRIDIZATION AND STRAND DISPLACEMENT WITH OLIGORIBONUCLEOTIDES, INTERACTION WITH RNASE-H AND HIV REVERSE-TRANSCRIPTASE, Chemistry, 3(9), 1997, pp. 1513-1520
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry
Journal title
Chemistry → ACNP
ISSN journal
0947-6539
Volume
3
Issue
9
Year of publication
1997
Pages
1513 - 1520
Database
ISI
SICI code
0947-6539(1997)3:9<1513:1O-HAS>2.0.ZU;2-D
Abstract
Hexitol nucleic acids (HNAs) with four natural bases form stable and s equence-selective duplexes with RNA. This was investigated by T-m dete rminations and gel shift experiments. The CD spectra of an HNA-RNA dup lex show similarities with the CD spectra of the A-form of dsRNA. Sing le-stranded HNAs are able to induce strand displacement in a double-st randed RNA sequence. An HNA-RNA duplex is a poor substrate for RNase H , and can inhibit the RNase H-mediated cleavage of a natural DNA-RNA s ubstrate. The HNA-RNA hybrid enhances the activity of HIV reverse tran scriptase.