THE EFFECT OF STRAIN OF DONOR EGGS, CULTURE CONDITIONS, AND EXPERIMENTAL PROTOCOL ON RELEASE OF EGGSHELL CALCIUM BY THE CHICK CHORIOALLANTOIC MEMBRANE IN-VITRO

Authors
Citation
Mj. Packard, THE EFFECT OF STRAIN OF DONOR EGGS, CULTURE CONDITIONS, AND EXPERIMENTAL PROTOCOL ON RELEASE OF EGGSHELL CALCIUM BY THE CHICK CHORIOALLANTOIC MEMBRANE IN-VITRO, Canadian journal of zoology, 75(9), 1997, pp. 1474-1478
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Zoology
Journal title
ISSN journal
0008-4301
Volume
75
Issue
9
Year of publication
1997
Pages
1474 - 1478
Database
ISI
SICI code
0008-4301(1997)75:9<1474:TEOSOD>2.0.ZU;2-3
Abstract
Factors affecting release of calcium by the chick chorioallantoic memb rane in vitro were assessed by culturing explants of shell with and wi thout the chorioallantoic epithelium. Explants were removed from eggs on day 16 of incubation and cultured using techniques similar to those used to examine resorption of bone in vitro. The region of an egg fro m which an explant is removed, the strain of donor eggs used, and addi tion of bovine serum albumin to the culture medium did not affect rele ase of calcium. In contrast, exposure of explants to a variety of diff erent culture media (DMEM, Medium 199, BGJ(b), and RPMI) introduced co nsiderable variation in calcium release. Calcium release in vitro was also examined by removing the blunt end of a fertile egg as well as th e embryo and yolk. The remaining preparation was filled with culture m edium, covered, and cultured for two 24-h periods, with a change of me dium after the first 24 h. Preparations from which the chorioallantoic membrane had been removed released very little calcium during culture , whereas those with the epithelium in place released considerable qua ntities. However, release of calcium by preparations with the membrane declined during the second interval. When surface area is taken into account, such preparations mobilize less calcium than explants culture d under similar conditions.