CHARACTERIZATION OF CU-RESISTANT BACTERIAL COMMUNITIES IN CU-CONTAMINATED SOILS

Citation
T. Kunito et al., CHARACTERIZATION OF CU-RESISTANT BACTERIAL COMMUNITIES IN CU-CONTAMINATED SOILS, Soil Science and Plant Nutrition, 43(3), 1997, pp. 709-717
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Agriculture,"Agriculture Soil Science
ISSN journal
0038-0768
Volume
43
Issue
3
Year of publication
1997
Pages
709 - 717
Database
ISI
SICI code
0038-0768(1997)43:3<709:COCBCI>2.0.ZU;2-E
Abstract
Bacterial communities in a spoil heap in a copper mine, a forest soil with high Cu contamination, and an agricultural soil with low Cu conta mination were characterized in terms of Gram-staining, plasmid frequen cy, pigmentation, Cu-resistance system, and predominant Cu-resistant b acterial species, Similarity existed in that the ratio of green coloni es for sorbing Cu and Gram-negative bacteria increased with the increa se of the Cu content of the medium regardless of the characteristics o f the bacterial communities, It was found that the plasmid was not pre sent in most of the Cu-resistant bacteria of the soils, Cu-resistant g enera including Burkholderia, Alcaligenes, and Methylobacterium specie s were isolated from the Cu contaminated soils using YG agar plates tr eated with 2 mM Cu. Furthermore, the bacterium with the highest Cu res istance (MIC = 5.5 mM Cu) was identified as a Gram-positive bacterium Bacillus sp., though most of the Cu-resistant bacteria were Gram-negat ive.