INTEGRATION OF PROVIRAL DNA INTO THE PDGF BETA-RECEPTOR GENE IN HTLV-I-INFECTED T-CELLS RESULTS IN A NOVEL TYROSINE KINASE PRODUCT WITH TRANSFORMING ACTIVITY

Citation
Kd. Chi et al., INTEGRATION OF PROVIRAL DNA INTO THE PDGF BETA-RECEPTOR GENE IN HTLV-I-INFECTED T-CELLS RESULTS IN A NOVEL TYROSINE KINASE PRODUCT WITH TRANSFORMING ACTIVITY, Oncogene, 15(9), 1997, pp. 1051-1057
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,Biology,"Cell Biology
Journal title
ISSN journal
0950-9232
Volume
15
Issue
9
Year of publication
1997
Pages
1051 - 1057
Database
ISI
SICI code
0950-9232(1997)15:9<1051:IOPDIT>2.0.ZU;2-I
Abstract
We have previously shown that noninfected human T-cell lines express t he canonical 5.7 kb mRNA coding for the type beta platelet-derived gro wth factor-receptor (PDGF beta-receptor), whereas HTLV-I-infected T-ce ll lines express a novel PDGF beta-receptor mRNA of 3.8 kb, In this re port, we have extended those studies to molecularly characterize the 3 .8 kb PDGF beta-receptor mRNA and show that it has resulted from integ ration of an apparently undeleted HTLV-I provirus into the PDGF beta-r eceptor gene in an orientation enabling expression of a truncated PDGF beta-receptor mRNA using the 3' HTLV-I long terminal repeat as a prom oter, Further, NIH3T3 cells transfected with a plasmid containing the truncated PDGF beta-receptor ORF plasmid generate colonies in soft aga r with more cells per colony than untransfected cells, or cells transf ected with the Tax 1 or PDGF-B (c-sis) plasmids, These results indicat e that the truncated PDGF beta-receptor protein acquires transforming capability and that HTLV-I-induced truncation of PDGF beta-receptor ma y correlate with HTLV-I-associated neoplasia of human T-cells.