PHOTOACTIVATION OF CAGED COMPOUNDS IN SINGLE LIVING CELLS - AN APPLICATION TO THE STUDY OF CELL LOCOMOTION

Citation
A. Ishihara et al., PHOTOACTIVATION OF CAGED COMPOUNDS IN SINGLE LIVING CELLS - AN APPLICATION TO THE STUDY OF CELL LOCOMOTION, BioTechniques, 23(2), 1997, pp. 268-274
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
0736-6205
Volume
23
Issue
2
Year of publication
1997
Pages
268 - 274
Database
ISI
SICI code
0736-6205(1997)23:2<268:POCCIS>2.0.ZU;2-O
Abstract
Caged compounds are molecules whose biological function is masked unti l UV light induces a photo-chemical reaction that converts the molecul es into a biologically active state. These probes provide very powerfu l tools in cell biology research, since the activation can be precisel y controlled temporally and spatially by limiting their exposure to li ght. This report describes a simple caged compound illuminator that ca n be attached to an inverted microscope; it can simultaneously provide both sufficient UV illumination for photoactivation and epifluorescen ce excitation to monitor indicator fluorescence. As an example, we sho w that the cytoplasmic calcium level can be raised very rapidly by the photoactivation of a new caged compound, caged calcium ionophore DMNP E-A23187, and that this [Ca2+](i) increase is associated with tail ret raction of motile keratocytes.