Detection of infectious enteroviruses and adenoviruses in tap water in urban areas in Korea

Authors
Citation
Sh. Lee et Sj. Kim, Detection of infectious enteroviruses and adenoviruses in tap water in urban areas in Korea, WATER RES, 36(1), 2002, pp. 248-256
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Environment/Ecology
Journal title
WATER RESEARCH
ISSN journal
0043-1354 → ACNP
Volume
36
Issue
1
Year of publication
2002
Pages
248 - 256
Database
ISI
SICI code
0043-1354(200201)36:1<248:DOIEAA>2.0.ZU;2-F
Abstract
We investigated the viral contamination of tap water at 11 urban sites in K orea between 1997 and 1998 over a period of 11 months. A total of 23 tap wa ter samples were examined for infectious enteroviruses and adenoviruses by a cell culture technique followed by polymerase chain reaction (PCR) amplif ication. To identify the recovered viruses, sequence analysis of PCR produc ts was performed. Infectious enteroviruses and adenoviruses were detected i n 11 (47.8%) and 9 (39.1%) of the samples, respectively. Both enteroviruses and adenoviruses were detected in five samples (21.7%). The level of viral contamination was quite high, ranging from 2 x 10(-3) to 2.9 x 10(-2) Most Probable Number of Infectious Unit L-1, far above the recommended virus le vel in drinking water set by the US EPA. Poliovirus type 1 derived from vac cine was frequently detected and the remainder comprised coxsackievirus B t ype or echovirus type 6, which were causative agents of aseptic meningitis in Korea in 1997 and 1998, respectively. Several types of adenovirus were d etected in tap water samples and some water samples were found to contain a denoviruses which were closely related to enteric adenovirus types 40 and 4 1. (C) 2001 Elsevier Science Ltd. All rights reserved.