Fluorescence study on the nyctinasty of Cassia mimosoides L. using novel fluorescence-labeled probe compounds

Citation
T. Sugimoto et al., Fluorescence study on the nyctinasty of Cassia mimosoides L. using novel fluorescence-labeled probe compounds, TETRAHEDRON, 57(49), 2001, pp. 9817-9825
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON
ISSN journal
0040-4020 → ACNP
Volume
57
Issue
49
Year of publication
2001
Pages
9817 - 9825
Database
ISI
SICI code
0040-4020(200112)57:49<9817:FSOTNO>2.0.ZU;2-X
Abstract
We synthesized fluorescence-labeled probe compounds bearing 6-((7-amino-4-m ethylcoumarin-3-acetyl)amino)-hexanoyl (AMCA, 1), 6-N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)-aminohexanoyl (NBD, 2), and 6-(4-((5-dimethylaminonaphthale ne-1-sulfonyl)amino))-hexanoyl (dansyl, 3) groups as the fluorescent functi onality. In these probe compounds, NBD-type probe, 2, showed leaf-opening a ctivity at 5 X 10(-6) M. The bioactivity of 2 is one-fifth as strong as tha t of the natural product, potassium isolespedezate (6). We carried out the binding experiment using 1 in a plant body. Then, it was suggested that flu orescence-labeled probe compound directly bound to a motor cell in pulvina of Cassia mimosoides. And this binding was specific to C. mimosoides. Probe compounds cannot bind plant sections of other nyctinastic plants. (C) 2001 Elsevier Science Ltd. All rights reserved.