Pretreatment with a deleted form of hepatocyte growth factor (DHGF) prevents the mortality of plasma-loss-induced hypovolemic shock in rats

Citation
H. Arisawa et al., Pretreatment with a deleted form of hepatocyte growth factor (DHGF) prevents the mortality of plasma-loss-induced hypovolemic shock in rats, SHOCK, 16(6), 2001, pp. 438-443
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aneshtesia & Intensive Care","Cardiovascular & Hematology Research
Journal title
SHOCK
ISSN journal
1073-2322 → ACNP
Volume
16
Issue
6
Year of publication
2001
Pages
438 - 443
Database
ISI
SICI code
1073-2322(200112)16:6<438:PWADFO>2.0.ZU;2-M
Abstract
Severe trauma, infection, burn, pancreatitis and major surgery often induce circulatory collapse leading to multiple organ failure and death. It is hy pothesized that therapy for the attenuation of circulatory collapse may imp rove the prognosis in these diseases. Previous work has documented that pre treatment with a deleted form of hepatocyte growth factor (dHGF) in normal rats increases the circulating plasma volume that reflects its accelerating action of hepatic protein synthesis. Therefore, the effects of pretreatmen t with dHGF on hypovolemic shock models were studied in rats. Rats were int ravenously administered dHGF (1 mg/kg, twice daily for 5-6 days) or vehicle , and subjected to a 25% total body surface area full-thickness burn or a t rypsin-induced acute pancreatitis, In rats that were receiving vehicle, sur vival rates on day 7 after injury induction were 12% in the burn model and 5% in the pancreatitis model, respectively. In both models, hematocrit valu es were apparently increased and circulating plasma volumes were decreased compared to sham-operated rats at 6 h after injury induction. The pretreatm ent of animals with dHGF increased the survival rates on day 7 to 40% in th e burn model and 29% in the pancreatitis model. dHGF-treatment in normal ra ts decreased the hematocrit values and increased the circulating plasma vol umes, and these changes of hematocrit value and circulating plasma volume w ere also maintained after injury induction. These findings suggest that dHG F pretreatment prevents the mortality in the severe burn and acute pancreat itis, and that its effect may contribute to ameliorating the progressing of plasma-loss-induced hypovolemia.