Isolation of a gene preferentially expressed in heart-shaped embryos of tobacco (Nicotiana tabacum)

Citation
St. Li et al., Isolation of a gene preferentially expressed in heart-shaped embryos of tobacco (Nicotiana tabacum), SEX PLANT R, 14(3), 2001, pp. 173-175
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
SEXUAL PLANT REPRODUCTION
ISSN journal
0934-0882 → ACNP
Volume
14
Issue
3
Year of publication
2001
Pages
173 - 175
Database
ISI
SICI code
0934-0882(200111)14:3<173:IOAGPE>2.0.ZU;2-Q
Abstract
We constructed two tobacco cDNA libraries from 200 microdissected undiffere ntiated globular proembryos and 150 just-differentiating heart-shaped embry os using the RT PCR technique. Through differential screening of the two cD NA libraries, a cDNA clone, designated THE3 (tobacco heart-shaped embryo 3) , was sequenced and analyzed. The deduced amino acid sequence encodes a bas ic protein (pI 10.88) of 40.6 kDa and contains a putative nuclear targeting signal sequence. Through the whole-mount in situ hybridization, the transc ript corresponding to THE3 gene shows preferential expression in heart-shap ed embryos but is weakly expressed in globular proembryos. The spatial and temporal expression of the THE3 gene suggests that it may play a role durin g proembryo differentiation.