Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs) - High-performance ion-exchange chromatographic analysis

Citation
F. Lesignoli et al., Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs) - High-performance ion-exchange chromatographic analysis, J CHROMAT A, 922(1-2), 2001, pp. 177-185
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
922
Issue
1-2
Year of publication
2001
Pages
177 - 185
Database
ISI
SICI code
0021-9673(20010713)922:1-2<177:RASDOD>2.0.ZU;2-N
Abstract
Peptide nucleic acids (PNAs) are oligonucleotide mimics containing a pseudo peptide chain, which are able to bind complementary DNA tracts with high af finity and selectivity. Two mixed-sequence PNA undecamers (1 and 2) were sy nthesized and their double-stranded adducts with the complementary oligonuc leotides (3 and 4) were revealed by the appearance of the corresponding pea k in anion-exchange HPLC. A DEAE column was used and elution was performed with aqueous Tris buffer (pH 8) and an ionic strength gradient (0-0.5 M NaC l). The same effect was not observed with non-complementary oligonucleotide s. The stability of the PNA-DNA adducts under the conditions used in the ch romatographic system was studied as a function of temperature. Furthermore, in competition experiments double-stranded oligonucleotides were challenge d by a PNA complementary to one strand: the formation of the PNA-DNA hybrid and the displacement of the non-complementary strand were observed with hi gh specificity. The results suggest a possible use of ion-exchange HPLC for studying PNA-DNA interactions, and indicate the efficiency of PNA probes i n the chromatographic analysis of DNA. (C) 2001 Elsevier Science B.V. All r ights reserved.