A role for nuclear lamins in nuclear envelope assembly

Citation
Ri. Lopez-soler et al., A role for nuclear lamins in nuclear envelope assembly, J CELL BIOL, 154(1), 2001, pp. 61-70
Citations number
60
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
154
Issue
1
Year of publication
2001
Pages
61 - 70
Database
ISI
SICI code
0021-9525(20010709)154:1<61:ARFNLI>2.0.ZU;2-1
Abstract
The molecular interactions responsible for nuclear envelope assembly after mitosis are not well understood. In this study, we demonstrate that a pepti de consisting of the COOH-terminal domain of Xenopus lamin 83 (LB3T) preven ts nuclear envelope assembly in Xenopus interphase extracts. Specifically, LB3T inhibits chromatin decondensation and blocks the formation of both the nuclear lamina-pore complex and nuclear membranes. Under these conditions, some vesicles bind to the peripheral regions of the chromatin. These "nonf usogenic" vesicles lack lamin B3 (LB3) and do not bind LB3T; however, "fuso genic" vesicles containing LB3 can bind LB3T, which blocks their associatio n with chromatin and, subsequently, nuclear membrane assembly. LB3T also bi nds to chromatin in the absence of interphase extract, but only in the pres ence of purified LB3. Additionally, we show that LB3T inhibits normal lamin polymerization in vitro. These findings suggest that lamin polymerization is required for both chromatin decondensation and the binding of nuclear me mbrane precursors during the early stages of normal nuclear envelope assemb ly.