Fishing out proteins that bind to titin

Citation
Jw. Sanger et Jm. Sanger, Fishing out proteins that bind to titin, J CELL BIOL, 154(1), 2001, pp. 21-24
Citations number
31
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
154
Issue
1
Year of publication
2001
Pages
21 - 24
Database
ISI
SICI code
0021-9525(20010709)154:1<21:FOPTBT>2.0.ZU;2-3
Abstract
Another giant protein has been detected in cross-striated muscle cells. Giv en the name obscurin, it was discovered in a yeast two-hybrid screen in whi ch the bait was a small region of titin that is localized near the Z-band. Obscurin is about 720 kD, similar in molecular weight to nebulin, but prese nt at about one tenth the level (Young et al., 2001). Like titin, obscurin contains multiple immunoglobulin-like domains linked in tandem, but in cont rast to titin it contains just two fibronectin-like domains. It also contai ns sequences that suggest obscurin may have roles in signal transduction. D uring embryonic development, its localization changes from the Z-band to th e M-band. With these intriguing properties, obscurin may not remain obscure for long.