Bordetella petrii sp nov., isolated from an anaerobic bioreactor, and amended description of the genus Bordetella

Citation
F. Von Wintzingerode et al., Bordetella petrii sp nov., isolated from an anaerobic bioreactor, and amended description of the genus Bordetella, INT J SY EV, 51, 2001, pp. 1257-1265
Citations number
58
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN journal
1466-5026 → ACNP
Volume
51
Year of publication
2001
Part
4
Pages
1257 - 1265
Database
ISI
SICI code
1466-5026(200107)51:<1257:BPSNIF>2.0.ZU;2-H
Abstract
A novel Bordetella species was isolated from an anaerobic, dechlorinating b ioreactor culture enriched from river sediment. The only strain, Se-1111R(T ) (= DSM 12804(T) = CCUG 43448(T)), for which the name Bordetella petrii is proposed, is designated the type strain of the novel species. Strain Se-11 11RT was isolated from the dechlorinating mixed culture due to its ability to anaerobically reduce selenate to elemental selenium. Comparative 16S rDN A sequence analysis showed a close relationship between Se-1111RT and membe rs of the genus Bordetella within the beta -Proteobacteria, This close memb ers of the genus Bordetella within the beta -Proteobacteria. This close phy logenetic relatedness was also reflected in several metabolic properties of Se-1111R(T), including its incapacity to utilize carbohydrates, by the hig h G+C content (63.8 mol%) of its DNA and by the presence of Q-8 as the majo r isoprenoid quinone. DNA-DNA hybridization experiments with type strains o f all species of the genus Bordetella and closely related species Achromoba cter xylosoxidans subsp. denitrificans provided further evidence for the as signment of strain Se-1111RT as a novel species of the genus Bordetella. Th is genus currently consists of seven aerobic species, all of which are know n to occur in close pathogenic, opportunistic or possibly commensal relatio nships with various host organisms. B. petrii is the first member of this g enus isolated from the environment and capable of anaerobic growth. The pro posal of the novel species and an emended description of the genus Bordetel la is presented.