Pseudonocardia kongjuensis sp nov., isolated from a gold mine cave

Citation
Sd. Lee et al., Pseudonocardia kongjuensis sp nov., isolated from a gold mine cave, INT J SY EV, 51, 2001, pp. 1505-1510
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN journal
1466-5026 → ACNP
Volume
51
Year of publication
2001
Part
4
Pages
1505 - 1510
Database
ISI
SICI code
1466-5026(200107)51:<1505:PKSNIF>2.0.ZU;2-W
Abstract
The taxonomic position of an isolate that was recovered from a gold mine ca ve near Kongju, Republic of Korea, was determined by 16S rDNA sequence stud ies and chemotaxonomic characterization. Comparative studies of 16S rDNA se quences indicated that this organism was phylogenetically related to member s of the genus Pseudonocardia, branching outside a cluster encompassing Pse udonocardia autotrophica and Pseudonocardia compacta, The affiliation to th e genus was also supported by the cell chemistry, which was represented by a type IV cell wall, MK-8(H-4) as the major menaquinone, a phospholipid typ e pill pattern (phosphatidylcholine as a diagnostic phospholipid) and a DNA G+C content of 71 mol%, The fatty acid profile contained saturated, unsatu rated and 10-methyl branched fatty acids, but tuberculostearic acid and hyd roxy fatty acids were not present. The isolate differed from its phylogenet ic neighbours in the presence of phosphatidylethanolamine, dodecanoate, 16- methylheptadecenoate and 16-methylheptadecanoate and the absence of phospha tidylinositol mannoside and phosphatidylmethylethanolamine. The unique comb ination of physiological properties, the cellular fatty acid profile and DN A-DNA hybridization data indicates that this organism is readily differenti ated from the type strains of all of the validly published species of the g enus Pseudonocardia, The name Pseudonocardia kongjuensis sp, nov. is propos ed for the type strain, LM 157(T) (= IMSNU 50583(T) = KCTC 9990(T) = DSM 44 525(T)).