Description of Tropheryma whipplei gen. nov., sp nov., the Whipple's disease bacillus

Citation
B. La Scola et al., Description of Tropheryma whipplei gen. nov., sp nov., the Whipple's disease bacillus, INT J SY EV, 51, 2001, pp. 1471-1479
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN journal
1466-5026 → ACNP
Volume
51
Year of publication
2001
Part
4
Pages
1471 - 1479
Database
ISI
SICI code
1466-5026(200107)51:<1471:DOTWGN>2.0.ZU;2-3
Abstract
A detailed characterization was performed of the Whipple's disease bacillus , strain Twist-Marseille(T), isolated from the cardiac valve of a patient w ith Whipple's disease bacillus endocarditis, This strain was isolated and m aintained on human embryonic lung fibroblast monolayers, but could not be c ultivated in the absence of living eukaryotic cells. Two morphological form s were observed, with differing staining properties; an intracellular form with intact and degenerating bacteria within vacuoles of infected cells and an extracellular form with masses of bacteria embedded in an extracellular matrix. Determination of the DNA G+C content confirmed that it belongs to the high-G+C Gram-positive bacteria, Strain Twist-Marseille(T) (= CNCM I-22 02(T)) is proposed as the type strain of a new species within a new genus, Tropheryma whipplei gen, nov., sp. nov., that was provisionally created sol ely on the basis of 16S rRNA gene sequence data.