Detection of amplified int-2/FGF-3 gene in primary breast carcinomas usingdifferential polymerase chain reaction

Citation
R. Naidu et al., Detection of amplified int-2/FGF-3 gene in primary breast carcinomas usingdifferential polymerase chain reaction, INT J MOL M, 8(2), 2001, pp. 193-198
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN journal
1107-3756 → ACNP
Volume
8
Issue
2
Year of publication
2001
Pages
193 - 198
Database
ISI
SICI code
1107-3756(200108)8:2<193:DOAIGI>2.0.ZU;2-U
Abstract
Amplification of int-2/FGF-3 gene was investigated by differential polymera se chain reaction (dPCR) in 440 archival primary breast carcinoma tissues. Of these, 23 were comedo ductal carcinoma in situ (DCIS), 18 were non-comed o DCIS, 41 were comedo DCIS with adjacent invasive ductal carcinomas, 19 we re non-comedo DCIS with adjacent invasive ductal carcinomas, 270 were invas ive ductal carcinomas, 33 were invasive lobular carcinomas, 21 were colloid carcinomas and 15 were medullary carcinomas. Int-2 was amplified in 22% (9 6/440) of the primary breast carcinomas. It was shown that int-2 was amplif ied in 13% (3/23) of the comedo DCIS, 17% (7/41) of the comedo DCIS and 29% (12/41) of the adjacent invasive ductal carcinomas, 26% (71/270) of the in vasive ductal carcinomas, 18% (6/33) of the invasive lobular carcinomas, 10 % (2/21) of the colloid carcinomas and 13% (2/15) of the medullary carcinom as. In contrast, int-2 was not amplified in non-comedo DCIS and invasive du ctal carcinomas with adjacent non-comedo DCIS lesions. A significant associ ation was observed between int-2 amplification in the in situ components an d adjacent invasive lesion (P <0.05). All tumors with int-2 amplification i n the in situ lesions (7/7) also demonstrated same degree of amplification in the adjacent invasive components. However, 9% (5/53) of the tumors with no amplified int-2 gene in the in situ components showed int-2 amplificatio n in the adjacent invasive lesions. A significant relationship was noted be tween amplification of int-2 and lymph node metastases (P <0.05) and poorly differentiated tumors (P <0.05) but not with estrogen receptor status (P > 0.05) and proliferation index (Ki-67 and PCNA) (P >0.05). In Malaysia, majo rity of the patients belong to younger age group (< 50 years old) but a com parison of the age groups showed that the amplification of int-2 was not st atistically associated with patient age (P >0.05). These observations indic ate that amplification of int-2 tends to strengthen the view that int-2 may have the potential to be an indicator of poor prognosis regardless of the age of the patient. Moreover, the presence of int-2 amplification in preinv asive, preinvasive and adjacent invasive lesions, and invasive carcinomas s uggest that int-2 could be a marker of genetic instability occurring in ear ly and late stages of tumor development.