Induction of T cell responses against autologous ovarian tumors with wholetumor cell lysate-pulsed dendritic cells

Citation
X. Zhao et al., Induction of T cell responses against autologous ovarian tumors with wholetumor cell lysate-pulsed dendritic cells, IMMUNOL INV, 30(1), 2001, pp. 33-45
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
IMMUNOLOGICAL INVESTIGATIONS
ISSN journal
0882-0139 → ACNP
Volume
30
Issue
1
Year of publication
2001
Pages
33 - 45
Database
ISI
SICI code
0882-0139(2001)30:1<33:IOTCRA>2.0.ZU;2-I
Abstract
The loading of dendritic cells (DCs) with whole tumor cell lysates may circ umvent the Facts that few tumor-specific antigens have been identified in h uman solid tumors. The present study was designed to investigate whether ov arian cancer cells lysate-pulsed DCs activate T cell responses against auto logous ovarian tumors. Incubation of T cells with autologous tumor cell lys ate-pulsed DCs stimulated proliferation of autologous T cells. T cells prim ed by autologous tumor cell lysate-pulsed DCs showed significant killing ac tivity against autologous tumor cells, which could be blocked by anti-MHC-c lass-I and anti-CD8 mAb. By contrast, T cells primed by autologous unpulsed DCs alone or tumor lysates alone failed to exhibit significant killing act ivity. In addition, T cells primed by DCs pulsed with allogeneic tumor cell lysates or with autologous normal cell lysate or by these cell lysates alo ne did not induce the increase in the autologous tumor killing activity. As additional controls, T cells stimulated with autologous tumor lysate-pulse d DCs express no increase in the lysis of autologous monocytes, allogeneic ovarian tumor cells and other cell lines including K562, Daudi and Molt-4. Furthermore, T cells stimulated with autologous tumor lysate-pulsed DCs cou ld produce the considerable amounts of cytokines such as GM-CSF. TNF-alpha and IFN-gamma. The data in the present study suggest that whole tumor cell lysates-pulsed DCs could activate T cell responses against autologous ovari an tumor cells. and that these pulsed DCs may be used as a new approach for the specific immunotherapy of ovarian cancer patients.