A novel mechanism of matrix metalloproteinase-9 gene expression implies a role for keratinization

Citation
T. Kobayashi et al., A novel mechanism of matrix metalloproteinase-9 gene expression implies a role for keratinization, EMBO REP, 2(7), 2001, pp. 604-608
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO REPORTS
ISSN journal
1469-221X → ACNP
Volume
2
Issue
7
Year of publication
2001
Pages
604 - 608
Database
ISI
SICI code
1469-221X(200107)2:7<604:ANMOMM>2.0.ZU;2-1
Abstract
To investigate the pathophysiological role of matrix metalloproteinase (MMP )-9 in the skin, we analyzed MMP-9 expression from human keratinocytes in c ulture. MMP-9 and the terminal differentiation marker involucrin were co-lo calized in the same keratinocytes with a high concentration of Ca2+, a pote nt stimulator of differentiation. We identified the novel KRE-M9 element, f urther downstream to the previously reported TPA responsive element in the MMP-9 promoter, and both of these two elements were shown to be important f or MMP-9 transcription and Ca2+ induction. The concomitant upregulation of MMP-9 and involucrin transcripts was probably due to the very similar gene regulatory elements, KRE-M9 and KRE-4 in their respective promoters. These results indicate a novel mechanism of transcriptional regulation for MMP-9 in the process of keratinization, implying the probable association of apop tosis and differentiation of keratinocytes in epidermal skin tissue.