C-type natriuretic peptide system in rabbit oviduct

Citation
Sh. Kim et al., C-type natriuretic peptide system in rabbit oviduct, PEPTIDES, 22(7), 2001, pp. 1153-1159
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PEPTIDES
ISSN journal
0196-9781 → ACNP
Volume
22
Issue
7
Year of publication
2001
Pages
1153 - 1159
Database
ISI
SICI code
0196-9781(200107)22:7<1153:CNPSIR>2.0.ZU;2-4
Abstract
C-type natriuretic peptide (CNP), a third member of the natriuretic peptide family, is known to be distributed mainly in brain and vascular endotheliu m and is considered to act as a local regulator in many tissues. The purpos e of this study was to determine the presence of CNP system and its biologi cal function in rabbit oviduct. The serial dilution curve of tissue extract s was parallel to the standard curve of CNP(1-22) and a major peak of molec ular profile of tissue extracts by HPLC: was CNP(1-53). mRNA of CNP which w as the same size as positive control was also detected by Southern blot ana lysis. CNP increased the production of 3',5'-cyclic guanosine monophosphate (cGMP) in the purified membrane of oviduct, which was more in membranes de rived from the isthmic portion than in the ampullar portion. The presence o f mRNAs of natriuretic peptide receptor-A (NPR-A) and NPR-B; was demonstrat ed by RT-PCR. Synthetic CNP(1-22) inhibited both frequency and amplitude of basal motility of oviduct in a dose-dependent manner. The inhibitory effec t of CNP on the basal motility was more potent in the isthmic portion than in the ampullar portion. These results demonstrate the presence of CNP syst em in the oviduct and regional differences in motility inhibition by CNP be tween isthmic and ampullar portions. Therefore, these findings suggest the possible existence of a CNP system that may exert a local regulator of basa l motility, either alone or in concert with other hormones. (C) 2001 Elsevi er Science Inc. All rights reserved.