Ubiquitin C-terminal hydrolase-L1 (PGP9.5) expression in human neural celllines following induction of neuronal differentiation and exposure to cytokines, neurotrophic factors or heat stress

Citation
Ji. Satoh et Y. Kuroda, Ubiquitin C-terminal hydrolase-L1 (PGP9.5) expression in human neural celllines following induction of neuronal differentiation and exposure to cytokines, neurotrophic factors or heat stress, NEUROP AP N, 27(2), 2001, pp. 95-104
Citations number
57
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
ISSN journal
0305-1846 → ACNP
Volume
27
Issue
2
Year of publication
2001
Pages
95 - 104
Database
ISI
SICI code
0305-1846(200104)27:2<95:UCH(EI>2.0.ZU;2-A
Abstract
Dysfunction of the ubiquitin-dependent proteolytic pathway contributes to p rogressive accumulation of ubiquitinated protein inclusions in neurodegener ative disorders, such as Parkinson's disease (PD), Ubiquitin C-terminal hyd rolase-L1 (UCH-L1). alternatively designated protein gene product 9.5 (PCP9 .5), is a neural deubiquitinating enzyme which is identified as a principal constituent of Lewy bodies. To clarify the regulatory mechanism of UCH-L1 expression in human neural cells, we studied the constitutive. cytokine/neu rotrophic factor-regulated, and heat stress-induced expression of UCH-L1 in cultured human neural cell lines by Western blot analysis. The constitutiv e expression of UCH-LI was identified in SK-N-SH neuroblastoma cells, IMR-3 2 neuroblastoma cells, U-373MG astrocytoma cells. and NTera2 teratocarcinom a-derived differentiated neurones (NTera2-N). The levels of UCH-L1 expressi on were unaltered in these cell lines following treatment with TNF-alpha, I L-1 beta, BDNF. GDNF, dibutyryl cyclic AMP, or phorbol 12-myristate 13-acet ate, and remained unchanged by exposure to heat stress. In contrast. its le vels were elevated substantially in NTera2 teratocarcinoma cells following retinoic acid-induced neuronal differentiation, accompanied with an increas ed expression of alpha -synuclein and synaptophysin, These results indicate that UCH-L1 is expressed constitutively in human neual cell lines, where i t is upregulated following induction of neuronal differentiation, but unaff ected by exposure to heat stress, cytokines. or growth/differentiation fact ors which are supposed to be involved in the nigral neuronal death and surv ival in PD.