p-nonylphenol acts as a promoter in the BALB/3T3 cell transformation

Authors
Citation
A. Sakai, p-nonylphenol acts as a promoter in the BALB/3T3 cell transformation, MUT RES-GTE, 493(1-2), 2001, pp. 161-166
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
ISSN journal
1383-5718 → ACNP
Volume
493
Issue
1-2
Year of publication
2001
Pages
161 - 166
Database
ISI
SICI code
1383-5718(20010627)493:1-2<161:PAAAPI>2.0.ZU;2-P
Abstract
p-Nonylphenol (NP) has attracted attention as an estrogenic contaminant, an d the environmental pollution by NP has been found to be extensive. NP is c lassified as a phenolic antioxidant based on the chemical activity and stru cture. Some phenolic antioxidants are known to induce and/or enhance carcin ogenesis. We examined the effects of NP on the two-stage transformation of BALB/3T3 cells, a model of two-stage carcinogenesis. The treatment by NP in the promotion phase markedly enhanced the transformation of the cells pre- treated with a subthreshold dose of a carcinogen, 3-methylcholanthrene (MCA ), but not that of non-pretreated cells. The promoting activity of NP was a pproximately one hundredth of that of 12-O-tetradecanoylphorbol-13-acetate (TPA), a potent tumor promoter, in the cell transformation. The treatment b y NP in the initiation phase did not induce cell transformation with and wi thout post-treatment by TPA. These results indicate that NP acts as a pure promoter of cell transformation implying that it may cause the enhancement of carcinogenesis in vivo. The enhancement by NP of MCA-initiated transform ation was suggested not to be mediated by estrogen receptors in BALB/3T3 ce lls because 17 beta -estradiol did not promote cell transformation in our e xperiments, and it has been reported that BALB/3T3 cells do not express est rogen receptors at a detectable level. (C) 2001 Elsevier Science B.V. All r ights reserved.