Helicobacter pylori DNA in drinking water in Japan

Citation
T. Horiuchi et al., Helicobacter pylori DNA in drinking water in Japan, MICROB IMMU, 45(7), 2001, pp. 515-519
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MICROBIOLOGY AND IMMUNOLOGY
ISSN journal
0385-5600 → ACNP
Volume
45
Issue
7
Year of publication
2001
Pages
515 - 519
Database
ISI
SICI code
0385-5600(2001)45:7<515:HPDIDW>2.0.ZU;2-Z
Abstract
Helicobacter pylori has been detected in drinking water in Peru and Sweden, suggesting the possibility of water-borne transmission. To date there have been few reports of H, pylori being detected in water; one was of the ureA gene of H, pylori in wells and springs in rural Japan. We examined water s ampled in or near urban areas of Japan for H, pylori DNA by three assays us ing the polymerase chain reaction (PCR), Near Tokyo, samples were obtained: 10 of tap water, 6 of well water 10 of river water, and 10 of sea water. S amples were filtered with membranes with 0.05- or 0.22-mum pores, which bac terial cells are caught by, Bacterial nucleic acids were extracted and puri fied and the PCR was done to amplify adhesin specific for H, pylori and the ureA gene, if present. Real-time PCR that measured the yield in terms of f luorescence was done with primers for 165 rRNA, None of the samples of tap, river, or sea water contained adhesin, ureA or 16S rRNA, None of the 6 sam ples of well water contained adhesin or ureA, but 2 of the 6 samples contai ned 16S rRNA, Some of the users of the well had had H, pylori infection in the past. H, pylori DNA was detected in well water and the users had been i nfected, so water-borne transmission via well water may occur even in towns in Japan.