Cholecystokinin characterization in urine by HPLC and immunoblotting

Citation
C. Massoubre et al., Cholecystokinin characterization in urine by HPLC and immunoblotting, J LIQ CHR R, 24(9), 2001, pp. 1365-1369
Citations number
6
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
9
Year of publication
2001
Pages
1365 - 1369
Database
ISI
SICI code
1082-6076(2001)24:9<1365:CCIUBH>2.0.ZU;2-4
Abstract
This paper describes a qualitative immunoreactivity cholecystokinin (CCK) c haracterization in 24 hours urine specimens. Urine was subjected to ethanol precipitation, followed by ion exchange chromatography, and immunoblotting with 2 antibodies. HPLC was developed to authenticate qualitatively the pr esence of CCK revealed by immunoblotting. The results obtained by HPLC showed this method was more specific. HPLC all owed us to distinguish, through anti-CCK reactive fractions, a single peak which reacted only with CCK antibodies and not with gastrin antibodies.