Simplified procedure for the determination of codeine and its metabolite in urine and plasma by LC/UV and LC/MS using mixed-mode cation exchange for sample preparation

Citation
Yf. Cheng et al., Simplified procedure for the determination of codeine and its metabolite in urine and plasma by LC/UV and LC/MS using mixed-mode cation exchange for sample preparation, J LIQ CHR R, 24(9), 2001, pp. 1353-1364
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
9
Year of publication
2001
Pages
1353 - 1364
Database
ISI
SICI code
1082-6076(2001)24:9<1353:SPFTDO>2.0.ZU;2-B
Abstract
Novel mixed-mode cation exchange (MCX) and HPLC methods were developed to d etermine codeine and its metabolite from human urine and porcine plasma. Fo r sample cleanup, spiked sample solutions were acidified followed by a well -thought-out MCX method using 3 cc, 60 mg Oasis(R) MCX cartridges. In this MCX method there is no need to condition and to equilibrate the sorbent; th e sample is loaded directly onto the sorbent. Recoveries from human urine a nd porcine plasma matrices were greater than 87% with RSDs less than 5.4%. For the HPLC analysis, the separation was obtained using a Symmetry(R) C-18 column with a simple mobile phase of 0.05% TFA/acetonitrile/methanol at 90 .5.5. Good peak shapes were obtained without the need of addition of any co mpeting reagent to the mobile phase.