Determination of quinine in drinks by reversed-phase ion-pair chromatography

Authors
Citation
Qc. Chen et J. Wang, Determination of quinine in drinks by reversed-phase ion-pair chromatography, J LIQ CHR R, 24(9), 2001, pp. 1341-1352
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
9
Year of publication
2001
Pages
1341 - 1352
Database
ISI
SICI code
1082-6076(2001)24:9<1341:DOQIDB>2.0.ZU;2-L
Abstract
A reversed-phase ion-pair chromatographic method was developed for the dete rmination of quinine in drinks. The separation was performed by an isocrati c elution with 0.4% glacial acetic acid-methanol (45:55, v/v), which contai ned 7.5 mmol/L sodium 1-heptanesulfonate as mobile phase (1 mL/min), and de tection by ultraviolet spectrometry at 236 nm and fluorimetry at 375 nm (wi th excitation at 334 nm). Good linearities between the concentrations of qu inine and relevant peak area responses were achieved in the range of 0.1-20 mg/mL by both detection modes. The detection limits (signal-to-noise ratio 3:1) for quinine were 0.02 mug/ mL and 0.004 mug/mL for spectrometric and fluorimetric detections, respecti vely. The method has been successfully applied to the analysis of commercia l drinks, and the average recoveries for various samples ranged from 91% to 106%.