Pressure effects in HPLC: Influence of pressure and pressure changes on peak shape, base line, and retention volume in HPLC separations

Authors
Citation
T. Macko et D. Berek, Pressure effects in HPLC: Influence of pressure and pressure changes on peak shape, base line, and retention volume in HPLC separations, J LIQ CHR R, 24(9), 2001, pp. 1275-1293
Citations number
54
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
9
Year of publication
2001
Pages
1275 - 1293
Database
ISI
SICI code
1082-6076(2001)24:9<1275:PEIHIO>2.0.ZU;2-V
Abstract
High sensitivity of modern detectors enables monitoring and utilisation of very small chromatographic effects caused by pressure. Till now, these effe cts have been often overlooked. Taking the influence of pressure on the HPL C system into account, numerous unexplained chromatographic phenomenona may be elucidated. Moreover, some pressure effects may be exploited for HPLC c olumn diagnostics, and pressure may even be employed to control retention b ehaviour of substances. Last but not least, ultrahigh pressure enables tran sporting eluent through long microparticulate column packings to reach extr emely high efficiency of HPLC separations.