D. Corradini et al., Fingerprinting of the development of aerobic composting processes of agricultural wastes by on-line combination of liquid chromatography and mass spectrometry, J LIQ CHR R, 24(9), 2001, pp. 1229-1244
Liquid chromatography (LC) was employed in conjunction to mass spectrometry
(MS) to develop a straightforward LC-MS procedure for monitoring the varia
tion of functionalized nonpolar organic compounds during the development of
an aerobic composting process of agricultural wastes. The LC separation wa
s performed by a reversed-phase microbore HPLC octadecyl-silica column, whi
ch was eluted by a linear acetonitrile gradient in water. The reversed-phas
e HPLC column was connected on-line to a triploquadrupole mass spectrometer
by an atmospheric pressure ionization interface.
Weighed amounts of agricultural wastes sampled at different time intervals
during the aerobic composting process were extracted by dichloromethane and
analyzed by LC-MS. The resulting fingerprinting offered a means to monitor
the variations of functionalized nonpolar compounds during the aerobic pro
cess and indicate the presence of compounds that are not degraded by the bi
otranformation, such as ursolic acid and beta -sitosterol.