Fingerprinting of the development of aerobic composting processes of agricultural wastes by on-line combination of liquid chromatography and mass spectrometry

Citation
D. Corradini et al., Fingerprinting of the development of aerobic composting processes of agricultural wastes by on-line combination of liquid chromatography and mass spectrometry, J LIQ CHR R, 24(9), 2001, pp. 1229-1244
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
9
Year of publication
2001
Pages
1229 - 1244
Database
ISI
SICI code
1082-6076(2001)24:9<1229:FOTDOA>2.0.ZU;2-M
Abstract
Liquid chromatography (LC) was employed in conjunction to mass spectrometry (MS) to develop a straightforward LC-MS procedure for monitoring the varia tion of functionalized nonpolar organic compounds during the development of an aerobic composting process of agricultural wastes. The LC separation wa s performed by a reversed-phase microbore HPLC octadecyl-silica column, whi ch was eluted by a linear acetonitrile gradient in water. The reversed-phas e HPLC column was connected on-line to a triploquadrupole mass spectrometer by an atmospheric pressure ionization interface. Weighed amounts of agricultural wastes sampled at different time intervals during the aerobic composting process were extracted by dichloromethane and analyzed by LC-MS. The resulting fingerprinting offered a means to monitor the variations of functionalized nonpolar compounds during the aerobic pro cess and indicate the presence of compounds that are not degraded by the bi otranformation, such as ursolic acid and beta -sitosterol.