Chemokine receptors in human basophils: inducible expression of functionalCXCR4

Citation
M. Iikura et al., Chemokine receptors in human basophils: inducible expression of functionalCXCR4, J LEUK BIOL, 70(1), 2001, pp. 113-120
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
JOURNAL OF LEUKOCYTE BIOLOGY
ISSN journal
0741-5400 → ACNP
Volume
70
Issue
1
Year of publication
2001
Pages
113 - 120
Database
ISI
SICI code
0741-5400(200107)70:1<113:CRIHBI>2.0.ZU;2-W
Abstract
We examined the expression profile of chemokine receptors in human basophil s and their regulation by cytokines, Basophils expressed transcripts of CC chemokine receptors (CCR)1, CCR2, CCRS, and CCR5 and CXC chemokine receptor s (CXCR)1, CXCR2, and CXCR4.. In contrast to the other receptors, surface-C XCR4 expression was not detected in fresh- and whole-blood basophils, but i t became apparent gradually during incubation, Among 16 chemokines tested, eotaxin induced the most potent basophil migration. SDF-1 also induced a st rong, migratory response comparable with that induced by eotaxin in 24-h, c ultured basophils, but it failed to induce degranulation, IL-3 abrogated CX CR4 expression completely, and it only down-regulated CCR2 and CCR3 express ion slightly, IL-5, GM-CSF, and IL-4 also down-regulated CXCR4 expression, Thus, expression of CXCR4 was the most strongly affected by cytokines, and this may represent an alternative mechanism for control of cell-specific, b iological responses to SDF-1.