Effect of AA-2414 on early and late bronchial responses in actively sensitized guinea-pigs

Citation
Y. Tohda et al., Effect of AA-2414 on early and late bronchial responses in actively sensitized guinea-pigs, J INT MED R, 29(3), 2001, pp. 229-235
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research General Topics
Journal title
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
ISSN journal
0300-0605 → ACNP
Volume
29
Issue
3
Year of publication
2001
Pages
229 - 235
Database
ISI
SICI code
0300-0605(200105/06)29:3<229:EOAOEA>2.0.ZU;2-E
Abstract
The effect of a new thromboxane A, receptor antagonist, AA-2414, (+/-)-7-(3 ,5,6-trimethyl-1,4-benzoquinon-2-yl)-7-phenyl-heptanoic acid, on dual bronc hoconstriction and airway hyper-reactivity in actively sensitized guinea-pi gs was investigated. Immediate and late bronchial responses were seen 1-10 min and 4-7 h, respectively, after inhalation of antigen. In guinea-pigs pr etreated with AA-2414, 5 mg/kg orally, the immediate bronchial response was inhibited. An administration of AA-2414 inhibited the late bronchial respo nse. The numbers of eosinophils, neutrophils and macrophages, but not of ly mphocytes, in bronchoalveolar lavage fluid were increased at 4 h after anti gen inhalation. AA-2414 did not affect the numbers of total cells, eosinoph ils, neutrophils or macrophages. Sensitized guinea-pigs showed a significan t airway hyperreactivity to inhaled histamine, which was not influenced by an administration of AA-2414. Luminol-dependent chemiluminescence of airway -infiltrated cells from sensitized guinea-pigs stimulated with A23187 was s lightly inhibited by AA-2414. These results show that AA-2414 inhibits the late asthmatic response and the production of oxygen radicals from airway-i nfiltrated cells.