Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis

Citation
Dm. Zhang et al., Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis, J CHROMAT B, 758(2), 2001, pp. 277-282
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
758
Issue
2
Year of publication
2001
Pages
277 - 282
Database
ISI
SICI code
1387-2273(20010715)758:2<277:AOTAAN>2.0.ZU;2-Q
Abstract
A simple but effective coupling of microdialysis and capillary electrophore sis with laser induced fluorescence detection technique was applied to anal ysis of amino acid neurotransmitters in the hypothalamus of rats after acut e exhausting exercise. The separation of amino acids was achieved using an uncoated fused-silica capillary (57 cm x 75 mum I.D.) with a buffer of 10 m M disodium tetraborate at pH 10 and an applied voltage of 12.5 kV. The dete ction limit was 10(-10) M for each amino acid. It is sufficiently sensitive and rapid for the determination of amino acids in a 5-mul Microdialysate. In comparison to pre-exercise, a significant increase in the levels of six hypothalamic amino acids (arginine, glycine, lysine, glutamic acid, alanine , gamma -amino-n-butyric acid) was found after exercise. These results demo nstrate that the increase of metabolic amino acids in the hypothalamus of r ats can be induced by exhausting exercise and suggests that amino acid neur otransmitters may play functional roles in the central effects of exercise. (C) 2001 Elsevier Science B.V. All rights reserved.