Sequence verification as quality-control step for production of cDNA microarrays

Citation
E. Taylor et al., Sequence verification as quality-control step for production of cDNA microarrays, BIOTECHNIQU, 31(1), 2001, pp. 62-65
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOTECHNIQUES
ISSN journal
0736-6205 → ACNP
Volume
31
Issue
1
Year of publication
2001
Pages
62 - 65
Database
ISI
SICI code
0736-6205(200107)31:1<62:SVAQSF>2.0.ZU;2-M
Abstract
To generate cDNA arrays in our core laboratory, we amplified about 2300 PCR products from a human sequence-verified cDNA clone library. As a quality-c ontrol step, rye sequenced the PCR products immediately before printing. Th e sequence information was used to search the GenBank((R)) database to conf irm the identities. Although these clones were previously sequence verified by the company, we found that only 79% of the clones matched the original database after handling. Our experience strongly indicates the necessity to sequence verify the clones at the final stage before printing on microarra y slides and to modify the gene list accordingly.