H. Tsumuki et al., Polyamine synthesis inhibitor, methylglyoxal bis (cyclopentylamidino hydrazone) (MGBCP) induces apoptosis in cultured rheumatoid synoviocytes and rheumatoid synovial tissue grafted into mice, BIOG AMINE, 16(3), 2001, pp. 269-284
To investigate effects of a polyamine synthesis inhibitor, methyl-glyoxal b
is (cyclopentylamidinohydrazone) (MGBCP), on the growth and induction of pr
ogrammed cell death (apoptosis) of both cultured rheumatoid synoviocytes in
vitro and the rheumatoid synovial tissue grafted into SCID mice in vivo.
MGBCP inhibited the growth of RA synoviocytes in a dose-dependent manner by
depletion of polyamine contents in the cells. Treatment of RA synoviocytes
with MGBCP induced apoptosis in the cultured synoviocytes. Administration
of MGBCP at two doses of 20 or 50 mg/kg into the grafted IIA tissue induced
apoptosis as demonstrated by morphological change and DNA fragmentation in
The present data suggest that artificial induction of apoptosis through the
injection of MGBCP may remove rheumatoid synovitis. These findings suggest
that the inhibition of polyamine synthesis results in the suppression of t
he growth of RA synoviocytes by inducing apoptosis in the human rheumatoid
synovial tissue in vitro and in vivo.