Intestinal absorption of rutin in free and conjugated forms

Citation
W. Andlauer et al., Intestinal absorption of rutin in free and conjugated forms, BIOCH PHARM, 62(3), 2001, pp. 369-374
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOCHEMICAL PHARMACOLOGY
ISSN journal
0006-2952 → ACNP
Volume
62
Issue
3
Year of publication
2001
Pages
369 - 374
Database
ISI
SICI code
0006-2952(20010801)62:3<369:IAORIF>2.0.ZU;2-A
Abstract
Quercetin is one of the most common flavonoids in nature, occurring mainly in glycosidic forms such as rutin. Rutin has been reported to exert numerou s biochemical and pharmacological activities, though information about its absorption and metabolism is scarce. The aim of this study was to investiga te intestinal handling of luminally administered rutin in an isolated prepa ration of luminally and vascularly perfused rat small intestine. A syntheti c perfusate free from blood components was used as vascular medium, with a perfluorocarbon as oxygen carrier. Luminal media consisted of a bicarbonate -buffered sodium chloride solution spiked with rutin (40.5 +/- 1.8 mu mol/L ). Viability was maintained during the entire perfusion; no differences bet ween rutin and control perfusions for perfusion pressure, lactate-pyruvate ratio, oxygen uptake, and acid-base homeostasis were observed. About 10% of the administered rutin appeared at the vascular side, chiefly as free ruti n (5.6%), but some rutin sulfate (2.5%) and glucuronide (2.0%) were also de tected. The conjugates were preferentially absorbed to the vascular side, w hile only traces of the glucuronide (0.2%) were found in the luminal perfus ate. Minute amounts of the rutin administered were located in the intestina l tissue (1.1%) in the form of unchanged rutin and its glucuronide and sulf ate conjugates. The model used serves as a valuable tool for understanding intestinal handling of the bioactive flavonol glycoside rutin, and the obta ined results confirm uptake of rutin ill the rat small intestine. (C) 2001 Elsevier Science Inc. All rights reserved.