Obtusifoliol 14 alpha-demethylase (CYP51) antisense Arabidopsis shows slowgrowth and long life

Citation
M. Kushiro et al., Obtusifoliol 14 alpha-demethylase (CYP51) antisense Arabidopsis shows slowgrowth and long life, BIOC BIOP R, 285(1), 2001, pp. 98-104
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
285
Issue
1
Year of publication
2001
Pages
98 - 104
Database
ISI
SICI code
0006-291X(20010706)285:1<98:O1A(AA>2.0.ZU;2-T
Abstract
Obtusifoliol 14 alpha -demethylase is a plant orthologue of sterol 14 alpha -demethylase (CYP51) essential in sterol biosynthesis, We have prepared CY P51 antisense Arabidopsis in order to shed light on the sterol and steroid hormone biosynthesis in plants. Arabidopsis putative CYP51 cDNA AtCYP51 was obtained from Arabidopsis expressed sequence tag (EST) library and its fun ction was examined in a yeast lanosterol 14 alpha -demethylase (Erg11) defi cient mutant. A recombinant AtCYP51 protein fused with a yeast Erg11 signal -anchor peptide was able to complement the erg11 mutation, which confirmed AtCYP51 to be a functional sterol 14 alpha -demethylase, AtCYP51 was then u sed to generate transgenic Arabidopsis by transforming with pBI vector harb oring AtCYP51 in the antisense direction under CaMV35S promoter. The result ing transgenic plants were decreased in accumulation of AtCYP51 mRNA and in creased in the amount of endogenous obtusifoliol, They showed a semidwarf p henotype in the early growth stage and a longer life span than control plan ts. This newly found phenotype is different from previously characterized b rassinosteroid (BR)-deficient campesterol biosynthesis mutants. (C) 2001 Ac ademic Press.