Strand exchange reaction in vitro and DNA-dependent ATPase activity of recombinant LIM15/DMC1 and RAD51 proteins from Coprinus cinereus

Citation
T. Nara et al., Strand exchange reaction in vitro and DNA-dependent ATPase activity of recombinant LIM15/DMC1 and RAD51 proteins from Coprinus cinereus, BIOC BIOP R, 285(1), 2001, pp. 92-97
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
285
Issue
1
Year of publication
2001
Pages
92 - 97
Database
ISI
SICI code
0006-291X(20010706)285:1<92:SERIVA>2.0.ZU;2-8
Abstract
We previously cloned recA-homolog genes from a basidiomycete, Coprinus cine reus, and obtained the recombinant proteins (Nara et at, Mot Gen, Genet. 26 2, 781-789, 1999, see Ref, 1; Nara and Sakaguchi, Biochem, Biophys. Res. Co mmun. 275, 97-102, 2000, see Ref, 2), The primary purpose of the present st udy was to characterize the biochemical properties of the recombinant LIM15 /DMC1 (CoLIM15) and RAD51 (CoRAD51) proteins. We purified the recombinant p roteins, and their molecular masses were 37 and 35 kDa, respectively. Both enzymes showed DNA-dependent ATPase activity and ATP-dependent strand excha nge reaction in vitro. CoRad51 was a five- to sixfold stronger DNA-dependen t ATPase and showed greater dependency on single-stranded DNA than CoLim15, In meiosis, both enzymes were highly accumulated in the meiotic tissue at leptotene and zygotene stages at which the homologous chromosomes pair, but disappeared just before the pachytene stage at which they recombine, From these and the previously reported results, we discuss here the relationship s between the enzymes and meiosis, (C) 2001 Academic Press.