Growth of the purple bacterium Rhodobacter capsulatus on the aromatic compound hippurate

Citation
Mt. Madigan et al., Growth of the purple bacterium Rhodobacter capsulatus on the aromatic compound hippurate, ARCH MICROB, 175(6), 2001, pp. 462-465
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF MICROBIOLOGY
ISSN journal
0302-8933 → ACNP
Volume
175
Issue
6
Year of publication
2001
Pages
462 - 465
Database
ISI
SICI code
0302-8933(200106)175:6<462:GOTPBR>2.0.ZU;2-V
Abstract
The purple nonsulfur bacterium Rhodobacter capsulatus strain B10 grew photo trophically on the aromatic compound hippurate (N-benzoyl-L-glycine) and re lated benzoyl amino acids. Absorption spectra, extraction, and GC/MS analys is of culture supernatants showed that hippurate was stoichiometrically con verted to benzoate and glycine, with the latter used as a carbon or nitroge n source for growth. This conclusion was supported by detection of the enzy me hippuricase in permeabilized intact cells. Chemotrophic growth on hippur ate by Rba. capsulatus, either at full or reduced oxygen tensions, was not observed. The type strain of Rhodobacter sphaeroides as well as four strain s of Rhodopseudomonas palustris also grew phototrophically on hippurate, wh ile several other aromatic-degrading species of purple bacteria did not.