Physical mapping: integrating computational and molecular genetic data

Citation
S. Le Hellard et al., Physical mapping: integrating computational and molecular genetic data, ANN HUM GEN, 65, 2001, pp. 221-228
Citations number
18
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Molecular Biology & Genetics
Journal title
ANNALS OF HUMAN GENETICS
ISSN journal
0003-4800 → ACNP
Volume
65
Year of publication
2001
Part
3
Pages
221 - 228
Database
ISI
SICI code
0003-4800(200105)65:<221:PMICAM>2.0.ZU;2-U
Abstract
A crucial step beyond the identification of genetic linkage of a disease to a chromosomal region is the production of a physical map that will allow t he identification of candidate genes. Although the process of physical map building has been facilitated by the flow of data released by the Human Gen ome. Project, gathering all the information together requires significant e ffort. In a previous study, we reported linkage between Bipolar Affective D isorder and the chromosomal location 4p15.3-p16.1. In this review we use th is example to describe how to collect publicly available sequence, DNA fing erprint, and genetic marker data and integrate these with empirical data to build a large scale high resolution physical map of a region. Methods used to identify new genetic markers and candidate genes within a circumscribed region are also presented.