Soluble Fas and Fas-ligand in bladder cancer in vitro and in vivo

Citation
Fge. Perabo et al., Soluble Fas and Fas-ligand in bladder cancer in vitro and in vivo, UROL ONCOL, 6(4), 2001, pp. 163-169
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Urology & Nephrology
Journal title
UROLOGIC ONCOLOGY
ISSN journal
1078-1439 → ACNP
Volume
6
Issue
4
Year of publication
2001
Pages
163 - 169
Database
ISI
SICI code
1078-1439(200107/08)6:4<163:SFAFIB>2.0.ZU;2-P
Abstract
Circulating soluble Fas (sFas) and expression of Fas-ligand on cancer cells are mechanisms of immune escape. The aim of the present study was to inves tigate expression and production of Fas and Fas-Ligand on bladder cancer ce ll lines of different grade as a basic mechanism of their secretion in vivo . sFas and sFas-ligand serum levels of patients with different stage of bla dder cancer were examined to determine the possible clinical use of these m olecules as tumor markers. Bladder cancer cell lints RT4 (G1). RT112 (G1), T24 (G3) and SUP (G4) were analyzed by flowcytometry for Fas and Fas-ligand expression. To determine if the fas-ligand gene is transcribed in these bl adder cancer cell lines, RT-PCR was performed on mRNA extracted from these cell lines. Production of sFas and sFas-ligand was examined in cell culture supernatants of the cancer cells as well as in the serum of 62 patients wi th bladder cancer by a specific ELISA test. We demonstrate that Fas is expr essed in similar levels on all human bladder carcinoma cell lines. In T24 ( G3) and SUP (G4) cell lines we were able to detect the Fas-ligand protein, whereas no Fas-ligand protein could be found in RT4 and RT112 (G1) cells. F as-ligand mRNA was expressed in all bladder cancer cell lines. Furthermore, all bladder cancer cell lines produce sFas but no sFas-ligand in spite of mRNA expression. The range of sFas levels in the serum of all patients with bladder cancer was large and did not show a correlation to the histopathol ogical stage of bladder cancer. Although there is in vitro evidence that sF as and Fas-ligand play a role in bladder cancer, no correlation between the sFas and sFas-ligand serum levels and the histopathological stage of bladd er cancer could be found. Therefore, serum sFas and sFas-ligand have to dat e limited clinical relevance. (C) 2001 Elsevier Science Inc. All rights res erved.