Karyotype of maize (Zea mays L.) mitotic metaphase chromosomes as revealedby fluorescence in situ hybridization (FISH) with cytogenetic DNA markers

Citation
Mt. Sadder et G. Weber, Karyotype of maize (Zea mays L.) mitotic metaphase chromosomes as revealedby fluorescence in situ hybridization (FISH) with cytogenetic DNA markers, PL MOL B RE, 19(2), 2001, pp. 117-123
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
PLANT MOLECULAR BIOLOGY REPORTER
ISSN journal
0735-9640 → ACNP
Volume
19
Issue
2
Year of publication
2001
Pages
117 - 123
Database
ISI
SICI code
0735-9640(200106)19:2<117:KOM(ML>2.0.ZU;2-X
Abstract
Here we demonstrate fluorescence in situ hybridization (FISH) of chromosome -specific cytogenetic DNA markers for chromosome identification in maize us ing repetitive and single copy probes. The fluorescently labeled probes, Ce ntC and pZm4-21, were shown to be reliable cytogenetic markers in the maize inbred line KYS for identification of mitotic metaphase chromosomes. The f luorescent strength of CentC signal, relative position, knob presence, size and location were used for the karyotyping. Based on direct visual analysi s of chromosome length and position of FISH signals, a metaphase karyotype was constructed for maize inbred line KYS. All chromosomes could be identif ied unambiguously. The knob positions in the karyotype agreed well with those derived from tra ditional cytological analyses except chromosomes 3, 4 and 8. One chromosome with a telomeric knob on the short arm was assigned to 3. A chromosome wit h a knob in the middle of the long arm was assigned number 4 by simultaneou s hybridization with a knob-specific probe pZm4-21 and a chromosome 4-speci fic probe Cent 4. On chromosome 8, we found an additional small telomeric k nob on the short arm. In addition, chromosome-specific probes were employed to identify chromosome 6 (45S rDNA) and chromosome 9 (single-copy probe um c105a cosmid).