Polymorphisms of GA/GT microsatellite loci from Anguilla japonica

Citation
Mc. Tseng et al., Polymorphisms of GA/GT microsatellite loci from Anguilla japonica, MAR BIOTEC, 3(3), 2001, pp. 275-280
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aquatic Sciences
Journal title
MARINE BIOTECHNOLOGY
ISSN journal
1436-2228 → ACNP
Volume
3
Issue
3
Year of publication
2001
Pages
275 - 280
Database
ISI
SICI code
1436-2228(200105/06)3:3<275:POGMLF>2.0.ZU;2-3
Abstract
Six novel microsatellite loci, containing (GA)(15-17) or (GT)(10-19) perfec t tandem repeats, were isolated and characterized for the catadromous eel A nguilla japonica. The allelic size of the 6 loci ranged from 79 to 226 bp i n length. All loci were polymorphic with a mean number of 14.7 alleles per locus and a mean heterozygosity of 0.67, suggesting higher polymorphism tha n that of freshwater and anadromous fishes, but lower than that of marine f ishes. Genotype diversity of the 6 loci ranged from 0.22 to 0.61 with a mea n value of approximately 0.5. Cross-species amplification showed that 5 of the 6 microsatellite primers proved to be useful in addressing questions of population genetics for all Anguilla species.