Comprehensive two-dimensional capillary LC and CE for resolution of neutral components in traditional Chinese medicines

Citation
Xm. Zhang et al., Comprehensive two-dimensional capillary LC and CE for resolution of neutral components in traditional Chinese medicines, J SEP SCI, 24(5), 2001, pp. 385-391
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF SEPARATION SCIENCE
ISSN journal
1615-9314 → ACNP
Volume
24
Issue
5
Year of publication
2001
Pages
385 - 391
Database
ISI
SICI code
1615-9314(200105)24:5<385:CTCLAC>2.0.ZU;2-2
Abstract
Comprehensive two-dimensional separation by capillary liquid chromatography coupled with capillary micellar electrokinetic chromatography was investig ated. Fast micellar electrokinetic chromatography was achieved by speeding up electroosmotic flow and improving solute mass transfer in micellar pseud o-stationary phase. High efficiency separation was achieved by selecting op erating conditions based on theoretical relationships and experimental data . A new gating interface from the first dimension to the second dimension b ased on effluent stacking-injection was developed. The reproducibility of t he interface for sample transfer efficiency, migration time, and peak heigh t was studied. The overall system performance was verified in the separatio n of complex neutral components. Resolution of hundreds of compounds presen t in traditional Chinese medicines was demonstrated.