Identification of mutans streptococcal species by the PCR products of the dex genes

Citation
T. Igarashi et al., Identification of mutans streptococcal species by the PCR products of the dex genes, J MICROB M, 46(2), 2001, pp. 99-105
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN journal
0167-7012 → ACNP
Volume
46
Issue
2
Year of publication
2001
Pages
99 - 105
Database
ISI
SICI code
0167-7012(200108)46:2<99:IOMSSB>2.0.ZU;2-K
Abstract
A pair of polymerase chain reaction (PCR) primers was designed on the basis of the nucleotide sequence homology of dextranase genes (dex) of Streptoco ccus mutans. S. sobrinus and S. downei. The primer pair amplified a 530-bp DNA fragment on the dex genes of mutans streptococcal species: S. mutants, S. sobrinus, S. downei. S. rattus and S. cricetus. HaeIII digestion of the 530-bp fragments generated species-specific subfragments. which were easily distinguishable from each other by agarose gel electrophoresis. These resu lts suggest that the PCR-amptification of the dex gene followed by the HaeI II digestion is useful for rapid identification of the five species of muta ns streptococci. (C) 2001 Elsevier Science B.V. All rights reserved.