Procedures allowing the transformation of a range of European elite wheat (Triticum aestivum L.) varieties via particle bombardment

Citation
S. Rasco-gaunt et al., Procedures allowing the transformation of a range of European elite wheat (Triticum aestivum L.) varieties via particle bombardment, J EXP BOT, 52(357), 2001, pp. 865-874
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
JOURNAL OF EXPERIMENTAL BOTANY
ISSN journal
0022-0957 → ACNP
Volume
52
Issue
357
Year of publication
2001
Pages
865 - 874
Database
ISI
SICI code
0022-0957(200104)52:357<865:PATTOA>2.0.ZU;2-7
Abstract
Ten current European wheat varieties were transformed at efficiencies rangi ng from 1-17% (mean 4% across varieties) following modifications in particl e bombardment and tissue culture procedures, All plants surviving phosphino thricin selection were screened for uidA and bar gene activity, and for the presence of marker gene sequences by PCR analysis, A minimum of 35% plant 'escape' frequency was achieved with selection on 4 mg l(-1) gluphosinate a mmonium after shoot initiation, Mean co-transformation frequency with vario us genes-of-interest was 66%. The estimated number of insertions of the uid A gene in 25 lines were; 1-2 in 32%, 3-5 in 52%, and 6-8 in 16% of lines. I n T-1 progenies, marker genes segregated in a Mendelian fashion in 50% of 3 9 lines analysed, as determined by transgene activity assays. Based on PCR analysis, it appeared that in some lines the occurrence of distorted segreg ation was due to poor transmission of the transgenes.