Oscillatory electroosmosis-enhanced intra/inter-particle liquid transport and its primary applications in the preparative electrochromatography of proteins

Citation
Z. Liu et al., Oscillatory electroosmosis-enhanced intra/inter-particle liquid transport and its primary applications in the preparative electrochromatography of proteins, J CHROMAT A, 921(1), 2001, pp. 93-98
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
921
Issue
1
Year of publication
2001
Pages
93 - 98
Database
ISI
SICI code
0021-9673(20010629)921:1<93:OEILTA>2.0.ZU;2-9
Abstract
The concept of generating an oscillatory electroosmotic flux inside the por ous particle to enhance the intra-particle mass transport was presented and a new kind of electrochromatography carried out in a five-compartment elec trolyzer were developed. The adsorbent was packed in the central compartmen t, while the neighboring compartments were used as the elution compartments and the electrode compartments, respectively. Chromatographic separations of human serum albumin on Blue Sepharose Fast Flow, bovine serum albumin (B SA) on DEAE-Sepharose Fast Flow, and BSA on hydroxyapatite were carried out , respectively. The adsorption isotherms were shown to be independent of el ectric field, while the increase in the electric field strength resulted in a linear increase in the magnitude of electroosmotic flux and the improvem ent of the breakthrough behavior in all cases. The experiment results have demonstrated the effectiveness of the oscillatory electroosmosis in enhanci ng intra- and inter-particle mass transport and its high potential to large -scale chromatography. (C) 2001 Elsevier Science B.V. All rights reserved.