RAPD, RFLP and SSLP analyses of phylogenetic relationships between cultivated and wild species of rice

Citation
Ns. Bautista et al., RAPD, RFLP and SSLP analyses of phylogenetic relationships between cultivated and wild species of rice, GENE GEN SY, 76(2), 2001, pp. 71-79
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Molecular Biology & Genetics
Journal title
GENES & GENETIC SYSTEMS
ISSN journal
1341-7568 → ACNP
Volume
76
Issue
2
Year of publication
2001
Pages
71 - 79
Database
ISI
SICI code
1341-7568(200104)76:2<71:RRASAO>2.0.ZU;2-E
Abstract
RAPD, RFLP, nuclear SSLP and chloroplast SSLP analyses were carried out to clarify the phylogenetic relationships among A-genome species of rice. In t otal, 12 cultivars of Oryza sativa (4 Japonica, 3 Javanica and 5 Indica), o ne cultivar of O. glaberrima, and 17 wild accessions (12 O. rufipogon, 2 O. glumaepatula, 1 O. longistaminata, 1 O. meridionalis and 1 O. barthii) wer e used. Their banding patterns were scored and compared to evaluate the sim ilarity between accessions. Genetic differentiation within and between taxa was examined based on the average similarity indices. Except for chloropla st SSLP analysis, the average similarities were higher within O. saliva tha n within O. rufipogon, and O. saliva Indica had greater intrasubspecific va riation than Japonica and Javanica. Comparisons between cultivated and wild species showed that O. saliva was closely related to O. rufipogon, while O . glaberrima was closely related to O. barthii. This indicated that two cul tivated species, O. saliva and O. glaberrimia, originated from O. rufipogon and O. barthii, respectively. Domestication of O. saliva seemed to be diph yletic, since strong similarity was observed between O. saliva Japonica-Jav anica and O. rufipogon from China and between (3. saliva Indica and O. rufi pogon from tropical Asia. In addition, dendrograms for RAPD, RFLP, and nucl ear and chloroplast SSLP analyses were constructed to reveal the overall ge netic relationships among A-genome species. In all analyses, O. saliva and O. glaberrima formed groups with O. rufipogon and O. barthii, respectively. However, their manners of clustering with other wild species were not the same. The results of RAPD and RFLP analyses indicate that O. glumaepatula w as relatively close to the groups of O. saliva and O. glaberrima whereas O. longistaminata and O. meridionalis were highly differentiated from other A -genome species. On the other hand, clear interspecific relationships were not obtained by nuclear or chloroplast SSLP analyses.