Membrane trafficking machinery components associated with the mammalian acrosome during spermiogenesis

Citation
J. Ramalho-santos et al., Membrane trafficking machinery components associated with the mammalian acrosome during spermiogenesis, EXP CELL RE, 267(1), 2001, pp. 45-60
Citations number
80
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
EXPERIMENTAL CELL RESEARCH
ISSN journal
0014-4827 → ACNP
Volume
267
Issue
1
Year of publication
2001
Pages
45 - 60
Database
ISI
SICI code
0014-4827(20010701)267:1<45:MTMCAW>2.0.ZU;2-U
Abstract
Active trafficking from the Golgi apparatus is involved in acrosome formati on, both by delivering acrosomal contents to the nascent secretory vesicle and by controlling organelle growth and shaping, During murine spermiogenes is, Golgi antigens (g-iantin, beta -COP, golgin 97, mannosidase II) are det ected in the acrosome until the late cap-phase spermatids, but are not foun d in testicular spermatozoa (maturation-phase spermatids), This suggests th at Golgi-acrosome flow may be relatively unselective, with Golgi residents retrieved before spermiation is complete. Treatment of spermatogenic cells with brefeldin A, a drug that causes the Golgi apparatus to collapse into t he endoplasmic reticulum, disrupted the Golgi in both pachytene spermatocyt es and round spermatids, However, this treatment did not affect the acrosom al granule, and some beta -COP labeling on the acrosome of elongating sperm atids was maintained. Additionally, N-ethylmaleimide sensitive factor, solu ble NSF attachment proteins, and homologues of the t-SNARE syntaxin and of the v-SNARE VAMP/synaptobrevin, as well as members of the rab family of sma ll GTPases, are associated with the acrosome (but not the acrosomal granule ) in round and elongated spermatids. This suggests that rab proteins and th e SNARE machinery for membrane recognition/docking/fusion may be involved i n trafficking during mammalian acrosome biogenesis, (C) 2001 Academic Press .