IMMUNOCYTOCHEMICAL LOCALIZATION OF THROMBOMODULIN IN THE AQUEOUS-HUMOR PASSAGE OF THE RAT EYE

Citation
T. Daimon et al., IMMUNOCYTOCHEMICAL LOCALIZATION OF THROMBOMODULIN IN THE AQUEOUS-HUMOR PASSAGE OF THE RAT EYE, HISTOCHEM C, 108(2), 1997, pp. 121-131
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology",Microscopy
Journal title
HISTOCHEMISTRY AND CELL BIOLOGY
ISSN journal
0948-6143 → ACNP
Volume
108
Issue
2
Year of publication
1997
Pages
121 - 131
Database
ISI
SICI code
0948-6143(1997)108:2<121:ILOTIT>2.0.ZU;2-O
Abstract
This report describes the distribution and localization of thrombomodu lin (TM) in the rat eye by light and electron microscopic immunocytoch emistry. In addition to the endothelium of the entire vasculature, TM was found on the non-vascular structures lining the cavities of the po sterior and anterior chambers and the limbus, TM was localized on the basal, lateral, and apical plasma membranes of the inner and outer cil iary epithelium, and the posterior iris epithelium in which there was no polarized expression of TM, In the anterior chamber, TM was localiz ed on the luminal surface of the corneal endothelium, but was negative on the anterior border layer of the iris, which is composed of a disc ontinuous layer of fibroblasts and collagen fibers. Thus, TM was prese nt at sites of cell-to-cell contact. TM was also present on the endoth elia of the trabecular meshwork and the Schlemm's canal in the limbus. TM was localized not only on the luminal plasma membrane, but also on the cytoplasmic giant vacuoles in the endothelial cells of the Schlem m's canal. These findings extend the importance of anticoagulant mecha nisms to the systems of secretion, circulation, and drainage of the aq ueous humor.