Quantitative evaluation of the lengths of homobifunctional protein cross-linking reagents used as molecular rulers

Authors
Green, NS Reisler, E Houk, KN
Citation
Ns. Green et al., Quantitative evaluation of the lengths of homobifunctional protein cross-linking reagents used as molecular rulers, PROTEIN SCI, 10(7), 2001, pp. 1293-1304
Citations number
135
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PROTEIN SCIENCE
ISSN journal
0961-8368 → ACNP
Volume
10
Issue
7
Year of publication
2001
Pages
1293 - 1304
Database
ISI
SICI code
0961-8368(200107)10:7<1293:QEOTLO>2.0.ZU;2-F
Abstract
Homobifunctional chemical cross-linking reagents are important tools for fu nctional and structural characterization of proteins. Accurate measures of the lengths of these molecules currently are not available, despite their w idespread use. Stochastic dynamics calculations now provide quantitative me asures of the lengths, and length dispersions, of 32 widely used molecular rulers. Significant differences from published data have been found.