Effect of hypothermia on brain cell membrane function and energy metabolism after transient global hypoxia-ischemia in the newborn piglet

Citation
Ws. Park et al., Effect of hypothermia on brain cell membrane function and energy metabolism after transient global hypoxia-ischemia in the newborn piglet, J KOR MED S, 16(3), 2001, pp. 335-341
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
General & Internal Medicine
Journal title
JOURNAL OF KOREAN MEDICAL SCIENCE
ISSN journal
1011-8934 → ACNP
Volume
16
Issue
3
Year of publication
2001
Pages
335 - 341
Database
ISI
SICI code
1011-8934(200106)16:3<335:EOHOBC>2.0.ZU;2-#
Abstract
This study was done to determine the effects of hypothermia on brain cell m embrane function and energy metabolism after transient hypoxia-ischemia (HI ) in the newborn piglet. Cerebral HI was induced by temporarily complete oc clusion of bilateral common carotid arteries with surgical dips and simulta neous breathing with 8% oxygen for 30 min, followed by release of carotid o cclusion and normoxic ventilation for 4 hr. Rectal temperature was maintain ed between 38.0 and 39.0 degreesC in normothermic groups, and between 34.0 and 35.0 degreesC in hypothermic groups for 4 hr after HI. During HI, heart rate, glucose and lactate level in the blood and cerebrospinal fluid incre ased, and base excess, pH and blood pressure decreased significantly in bot h normothermic and hypothermic groups. After HI, these abnormalities return ed to normal in normothermic group, but lactic acidosis persisted in hypoth ermic group. Decreased cerebral Na+,K(+)ATPase activity and increased lipid peroxidation products, indicative of HI-induced brain injury, were more pr ofound in hypothermic group than in normothermic group. Brain ATP and phosp hocreatine levels were not different between normothermic and hypothermic g roups. In summary, hypothermia applied immediately after HI for 4 hr did no t improve the recovery of brain cell membrane function and energy metabolis m in the newborn piglet.