Synergistic effects of highly unsaturated fatty acid-containing phosphatidylethanolamine on differentiation of human leukemia HL-60 cells by dibutyryl cyclic adenosine monophosphate

Citation
M. Hosokawa et al., Synergistic effects of highly unsaturated fatty acid-containing phosphatidylethanolamine on differentiation of human leukemia HL-60 cells by dibutyryl cyclic adenosine monophosphate, JPN J CANC, 92(6), 2001, pp. 666-672
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
JAPANESE JOURNAL OF CANCER RESEARCH
ISSN journal
0910-5050 → ACNP
Volume
92
Issue
6
Year of publication
2001
Pages
666 - 672
Database
ISI
SICI code
0910-5050(200106)92:6<666:SEOHUF>2.0.ZU;2-W
Abstract
Highly unsaturated fatty acid-containing phospholipid (HUFA-PL) has many nu tritional and medical applications. We investigated the effect of HUFA-PL o n differentiation of human leukemia HL-60 cells induced by dibutyryl cyclic adenosine monophosphate (dbcAMP). HUFA-containing phosphatidylethanolamine (HUFA-PE), such as salmon testis PE, significantly enhanced dbcAMP-induced cell differentiation. A combined treatment of 200 muM dbcAMP with 50 muM H UFA-PE increased the nitroblue tetrazolium (NBT)-reducing activity, which i s an indicator of differentiation, to a level comparable to that in the cas e of 500 muM dbcAMP treatment. In contrast, HUFA-lyso PE (a monoacyl form) did not exert an enhancing effect on dbcAMP-induced differentiation. The en hancing effect of HUFA-PE was suppressed by a protein kinase C inhibitor, s taurosporine, while a protein kinase A inhibitor, H.8, did not suppress the enhancing effect. These findings suggest that HUFA-PE might enhance dbcAMP -induced differentiation through modulation of the protein kinase C signali ng pathway in HL-60 cells.